Efecto del flameo en Trichoderma harzianum inoculado al suelo.
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2021-12
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Universidad EARTH
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Se evaluó el efecto del flameo en Trichoderma harzianum inoculado al suelo, mediante el conteo de Unidades Formadoras de Colonias (UFC) en laboratorio durante ocho días. Se inoculó una suspensión de Trichoderma harzianum en cuatro diferentes áreas experimentales (repeticiones) en un sistema agroforestal de cacao (Theobroma cacao) y un mes después se aplicó el flameo, se tomaron muestras de suelo una hora (1h), cuarenta y ocho horas (48h) y días (5d) posterior a la aplicación del tratamiento. Luego se aislaron las cepas de cultivo en dos diferentes diluciones: 1:1000 y 1:10000, mediante el uso del Medio Selectivo para Trichoderma harzianum (THSM). La cantidad de UFC contabilizadas no representaron una diferencia estadísticamente significativa (p>0.05) en ninguna de las dos diluciones estudiadas, 1:1000 y 1:10000, entre los datos obtenidos en el muestreo realizado antes de aplicado el tratamiento y una hora (1h) después de aplicado el tratamiento. Sin embargo, la cantidad de UFC contabilizadas cuarenta y ocho horas (48h) y cinco días (5d) después de aplicado el tratamiento fueron mayores, mostrando diferencias significativas (p<0.05) en comparación a los datos iniciales obtenidos. Se concluyó que el flameo no afecta al Trichoderma harzianum inoculado al suelo y que la mejor dilución para realizar este estudio fue la 1:10000 por permitir el conteo de UFC en laboratorio por más días consecutivos.
The effect of flaming in Trichoderma harzianum inoculated to the ground was evaluated by counting Colony Forming Units (CFU) in the laboratory for eight days. A suspension of Trichoderma harzianum was inoculated in four different experimental areas (repetitions) in a cocoa agroforestry system (Theobroma cacao) and a month later the flaming was applied, soil samples were taken one hour (1h), 48 hours (48h) and days (5d) after the application of the treatment. Then the culture strains were isolated in two different dilutions: 1:1000 and 1:10000, by using the Selective Medium for Trichoderma harzianum (THSM). The amount of counted CFUs did not represent a statistically significant difference (p>0.05) in any of the two dilutions studied, 1:1000 and 1:10000, between the data obtained in the sampling carried out before applying the treatment and one hour (1h) after the treatment is applied. However, the number of CFUs counted forty-eight hours (48h) and five days (5d) after applying the treatment were higher, showing significant differences (p<0.05) compared to the initial data obtained. It was concluded that flaming does not affect Trichoderma harzianum inoculated to the soil and that the best dilution to carry out this study was 1:10000 because it allowed the counting of CFU in the laboratory for more consecutive days.
The effect of flaming in Trichoderma harzianum inoculated to the ground was evaluated by counting Colony Forming Units (CFU) in the laboratory for eight days. A suspension of Trichoderma harzianum was inoculated in four different experimental areas (repetitions) in a cocoa agroforestry system (Theobroma cacao) and a month later the flaming was applied, soil samples were taken one hour (1h), 48 hours (48h) and days (5d) after the application of the treatment. Then the culture strains were isolated in two different dilutions: 1:1000 and 1:10000, by using the Selective Medium for Trichoderma harzianum (THSM). The amount of counted CFUs did not represent a statistically significant difference (p>0.05) in any of the two dilutions studied, 1:1000 and 1:10000, between the data obtained in the sampling carried out before applying the treatment and one hour (1h) after the treatment is applied. However, the number of CFUs counted forty-eight hours (48h) and five days (5d) after applying the treatment were higher, showing significant differences (p<0.05) compared to the initial data obtained. It was concluded that flaming does not affect Trichoderma harzianum inoculated to the soil and that the best dilution to carry out this study was 1:10000 because it allowed the counting of CFU in the laboratory for more consecutive days.
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CONTROL DE MALEZAS, CACAO, THEOBROMA CACAO, INOCULACION DEL SUELO