Efecto de luz ultravioleta tipo C para el control de patógenos causantes de la pudrición de corona del banano.
Fecha
2017-12
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Universidad EARTH
Resumen
El objetivo de la presente investigación fue evaluar el uso de luz ultravioleta tipo C para el control de patógenos causantes de la pudrición de corona del banano. La investigación se dividió en tres ensayos. En el ensayo 1 se evaluó la dosis máxima de aplicación de luz ultravioleta tipo C que no genera daños en la estética y calidad de la fruta expuesta en vida verde. En el ensayo 2 se determinó la dosis máxima de aplicación de luz ultravioleta tipo C que no genera daños en la estética y calidad de la fruta madura. Para el ensayo 3 se evalúo la eficacia de la luz ultravioleta tipo C sobre control de patógenos causantes de la pudrición de corona del banano simulando el transporte a 15 y 21 días, y una semana de maduración y vida en anaquel de la fruta. Para el ensayo 3 los clústeres de banano fueron inoculados con una suspensión de esporas de Fusarium y Colletotrichum a una concentración de 4x104 ufc/mL. Cuatro tratamientos fueron evaluados: dosis de luz UV-C de 0.0044 J/cm2 con 4 s de exposición y 0.0022 J/cm2 con 2 s, un tratamiento comercial utilizado en la planta empacadora de la Universidad EARTH y un testigo. Los resultados indicaron que tratamientos con exposiciones de luz ultravioleta superiores a 0.011 J/cm2 causan daños considerables en la calidad y estética de la fruta por lo tanto se descartan completamente para el uso comercial. El mejor tratamiento fue la dosis 0.0044 J/cm2 ya que presento el menor índice de pudrición de corono y fue estadísticamente semejante al tratamiento comercial. Por otro lado, el tratamiento menos efectivo fue de la dosis de 0.0022 J/cm2 ya que presento un índice de pudrición de corona mayor y similar estadísticamente al control. Los resultados de esta investigación indican el potencial del uso de la luz ultravioleta para el control de la pudrición de corona. Sin embargo, más investigaciones son necesarias para determinar la dosis y el tiempo de exposición ideal que no dañe la calidad de la fruta y tenga un control eficiente de la enfermedad.
The objective of the following research was to evaluate the use of ultraviolet light type C in the control of pathogens that cause banana crown rot. The research was divided into three experiments. The first trial, was to determine the maximum dose of application of ultraviolet light type C that does not cause damage to the cosmetic and quality of the fruit during the green life. The objective of trial two was to determine, the maximum dose of application of ultraviolet light type C, which does not cause damage to the cosmetic and quality of the ripening fruit. The objective of the trial three was to evaluate the effect of ultraviolet light type C on the control of pathogens causing banana crown rot, simulating 15 and 21 days of transportation, and one week of ripening and shelf life of the fruit. For trial 3, banana clusters were inoculated with a spore suspension of Fusarium and Colletotrichum at a concentration of 4X10X4 ufc/mL. Four treatments were evaluated: Doses of UV-C light. 0.0044 J/cm2 for 4 s exposure and 0.0022 J/cm2 for 2 s; a commercial treatment used in the packing plant at EARTH University and a control. The results indicated that treatments with higher exposure of 0.011 J/cm2 caused considerable damage in the quality and cosmetic appearance of the fruit, therefore this completely rejected for commercial use. The best treatment was doses 0.0044 J/cm2, due to presented the lowest crow rot index and it was statistical similar to the commercial treatment used in the packing station. On the other hand, the treatment less effective was the dose of 0.0022 J/cm2 due to presented higher value of crown rot index similar to the control, so that, can be not used as commercial treatment. The results of the present research indicated that the potential use of ultraviolet light type C can be used for the control of crown rot of banana fruit. However more research is needed to determine the time of exposure and the best doses which cannot affect the cosmetic appearance and the quality of the fruit as well as the efficiency on controlling the disease.
The objective of the following research was to evaluate the use of ultraviolet light type C in the control of pathogens that cause banana crown rot. The research was divided into three experiments. The first trial, was to determine the maximum dose of application of ultraviolet light type C that does not cause damage to the cosmetic and quality of the fruit during the green life. The objective of trial two was to determine, the maximum dose of application of ultraviolet light type C, which does not cause damage to the cosmetic and quality of the ripening fruit. The objective of the trial three was to evaluate the effect of ultraviolet light type C on the control of pathogens causing banana crown rot, simulating 15 and 21 days of transportation, and one week of ripening and shelf life of the fruit. For trial 3, banana clusters were inoculated with a spore suspension of Fusarium and Colletotrichum at a concentration of 4X10X4 ufc/mL. Four treatments were evaluated: Doses of UV-C light. 0.0044 J/cm2 for 4 s exposure and 0.0022 J/cm2 for 2 s; a commercial treatment used in the packing plant at EARTH University and a control. The results indicated that treatments with higher exposure of 0.011 J/cm2 caused considerable damage in the quality and cosmetic appearance of the fruit, therefore this completely rejected for commercial use. The best treatment was doses 0.0044 J/cm2, due to presented the lowest crow rot index and it was statistical similar to the commercial treatment used in the packing station. On the other hand, the treatment less effective was the dose of 0.0022 J/cm2 due to presented higher value of crown rot index similar to the control, so that, can be not used as commercial treatment. The results of the present research indicated that the potential use of ultraviolet light type C can be used for the control of crown rot of banana fruit. However more research is needed to determine the time of exposure and the best doses which cannot affect the cosmetic appearance and the quality of the fruit as well as the efficiency on controlling the disease.
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PATOGENESIS, BANANOS, RADIACION ULTRAVIOLETA